Regulatory

Part:BBa_K1328000:Design

Designed by: Tianyi Li   Group: iGEM14_Tsinghua   (2014-10-09)


HXT1-CMVmini


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Region -750~-161 bp was selected from the HXT1 promoter for maximal glucose sensitivity. A CMV-mini promoter sequence was added to increase expression rate.


Source

HXT1 promoter sequence is cloned from S. cerevisiae genome via PCR. The CMV-mini promoter is cloned from a commercial plasmid.

References